Part:BBa_K801093:Design

4-coumarate--coenzyme A ligase (4CL) coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1086
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1653
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1285
  

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/609339/

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, Phenylalanine Ammonia Lyase

Abbreviations: 4CL = 4-coumarate-coenzyme A Ligase

Design Notes

Related BioBrick:

Other versions: BBa_K801092: 4CL + yeast consensus sequence

Related BioBricks:

• BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence
• BBa_K801091: phenylalanine ammonia lyase (PAL) coding region
• BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
• BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
• BBa_K801095: Naringenin - chalcone synthase (CHS) coding region
• BBa_K801096: Aromatic prenyltransferase (APT) coding region
• BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence
• BBa_K801098: O-methyltransferase 1 (OMT1) coding region

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:
Protein: 4-coumarate-coenzyme A ligase

Enzymatic activity:
EC-number: 6.2.1.12

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Arabidopsis thaliana

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/U18675.1 GenBank: Arabidopsis thaliana 4-coumarate--coenzyme A ligase (At4CL1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q42524 Uniprot: 4-coumarate--CoA ligase 1]